Plasmid

Part:BBa_K4392004:Design

Designed by: Vikram Venugopal   Group: iGEM22_IISER-Pune-India   (2022-10-01)


pUC57-acdS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 396
    Illegal suffix found in sequence at 1433
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal SpeI site found at 1434
    Illegal PstI site found at 1448
    Illegal NotI site found at 402
    Illegal NotI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BamHI site found at 1285
    Illegal XhoI site found at 602
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 396
    Illegal suffix found in sequence at 1434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 396
    Illegal XbaI site found at 411
    Illegal SpeI site found at 1434
    Illegal PstI site found at 1448
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 625
    Illegal SapI site found at 1684


Design Notes

The acdS gene was inserted into this plasmid with a BioBrick prefix and suffix flagging it.


Source

This part was obtained through de novo gene synthesis.

References